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. 2016 Dec 19;9:648. doi: 10.1186/s13071-016-1928-0

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Immunohistochemistry analysis of IgA-secreting cells in the intestinal epithelium of orally immunized mice. IgA-secreting cells were stained dark brown. The jejuna (approximately 5–7 mm) of each group were isolated and submitted to immunohistochemical staining at week 4. Panels (a) and (b) represent PBS-treated mice. Panels (c) and (d) represent B.s-CotC orally administered mice. Panels (e) and (f) represent BL21-CsCP gavaged mice. Panels (g) and (h) represent mice orally administered with spores expressing CotC-CsCP. Scale-bars: a, c, e, g, 200 μm; b, d, f, h, 50 μm. The arrows indicate IgA-secreting cells. i Integrated option density (IOD) of IgA-secreting cells. ***P < 0.001