Fig. 5. SPFS kinetic scan curve collected during ligand addition and subsequent rinsing with buffer (start of rinsing is indicated by arrows) for a Cy5-labeled non-binding (flavin-free) ssDNA ligand (A), a Cy5-labeled flavin-dsDNA ligand (B), and a Cy5-labeled flavin-ssDNA ligand (C) measured on the same dodecin-DNA-layer.
