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. 2016 Dec 14;7:13692. doi: 10.1038/ncomms13692

Figure 5. TOC1 and PRR5 mediate the circadian gating of thermomorphogenesis.

Figure 5

(a,b) Effects of warm temperature at different ZTs on the expression of PIF4 (a) and YUC8 (b). Seedlings were entrained in 12 h light and 12 h dark (12L:12D) cycles at 20 °C for 4 days and then transferred to continuous light. At different ZTs, the growth temperature was increased to 29 °C or kept at 20 °C for 4 h. Seedlings were then harvested at ZT4, ZT16 and ZT20 for RNA extraction. Gene expression levels quantified by qRT–PCR were normalized to PP2A and to that of wild type at ZT0 and 20 °C. Grey dots in b indicate the ratio of YUC8 expression levels between 29 °C and 20 °C samples. Error bars indicate s.d. (n=3). *P<0.05 (Student's t-test).