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. 2016 Dec 14;7:13692. doi: 10.1038/ncomms13692

Figure 6. PIF4 is required for thermo-adaptation.

Figure 6

(a,b) PIF4 is required for thermo-adaptation. Seedlings of WT, pif4 and PIF4-OX genotypes were grown at 20 °C or 29 °C under the continuous light and then were subjected to a 45 °C heat shock for different time periods. After recovery under the constant light at 20 °C for 5 days, survival rate was measured for over 40 seedlings in each sample. The experiments were repeated for three times. Error bars indicate s.d. (n=3). *P<0.05 (Student's t-test). (c) The survival rate of WT, TOC-OX and toc1;prr5 seedlings grown at 20 °C or 29 °C, after 45 °C heat-shock treatment for the indicate time periods. Error bars indicate s.d. (n=3). *P<0.05 (Student's t-test). (d) A model of the circadian gating of thermomorphogenesis through the TOC1–PIF4 interaction. During the day, warm temperature activates PIF4, which in turn activates auxin biosynthesis genes including YUC8 and promotes hypocotyl growth. However, in the evening and at early night, TOC1 accumulates at high levels and directly inhibits PIF4, suppressing thermomorphogenesis.