Table 1. Details of the 16 microsatellite markers developed and optimized.

Locus ID	NA	Range	HO	HE	PIC	NAF	GenBank Acc. Nº
D.tru2	18	97–148	0.590	0.830	0.809	0.133	HG792255
D.tru4	14	151–179	0.641	0.750	0.716	0.123	HG792256
D.tru6	29	69–133	0.408	0.796	0.777	0.327	HG792257
D.tru8	14	127–169	0.670	0.823	0.796	0.109	HG792258
D.tru11	26	97–153	0.469	0.715	0.700	0.211	HG792259
D.tru14	19	75–135	0.207	0.557	0.534	0.540	HG792260
D.tru15	33	215–279	0.592	0.947	0.942	0.218	HG792261
D.tru16	26	70–124	0.768	0.912	0.903	0.093	HG792262
D.tru19	28	126–184	0.542	0.935	0.929	0.223	HG792263
D.tru22	12	215–249	0.280	0.689	0.639	0.410	HG792264
D.tru23	33	101–167	0.738	0.835	0.825	0.066	HG792265
D.tru26	28	55–119	0.590	0.924	0.917	0.223	HG792266
D.tru29	21	262–322	0.261	0.826	0.808	0.487	HG792268
D.tru32	25	196–284	0.323	0.674	0.659	0.425	HG792271
D.tru40	23	119–167	0.578	0.889	0.878	0.229	HG792273
D.tru49	15	144–182	0.238	0.801	0.771	0.553	HG792275

514 individuals from 7 sampling sites were used for these analyses. Locus identity, (N_A) number of alleles found, (Range) range of allele sizes, (H_O) observed and (H_E) expected heterozygosity, (PIC) polymorphism information content, (NAF) null-allele frequency and GenBank accession numbers.