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. 2016 Dec 19;6:39457. doi: 10.1038/srep39457

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Copyright © 2016, The Author(s)

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(a) Sox17-Cre × R26-tauGFP41 E5.5 embryo and post-XEN cell line. From left to right: whole E5.5 embryo, large outgrowth of embryo after 5 days in culture, cells after outgrowth disaggregation and culture for 11 days, and established post-XEN cell line X-E5.5-8 on day 22. Combined intrinsic green fluorescence of GFP/bright-field image. (b) Fluorescence analysis of post-XEN cell line X-E5.5-10. First column: intrinsic (indicated with an asterisk after GFP) green fluorescence of GFP from the ROSA26 locus after activation by Cre recombinase that is expressed from the gene-targeted Sox17 locus. Second column: cells are immunoreactive (magenta) for XEN markers GATA4, GATA6, SOX7, and SOX17. Third column: cells are stained for XEN marker DAB2, but not for ES cell markers OCT4 and NANOG, and not for TS cell marker CDX2. Fourth column: DAPI (blue).