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. 2016 Dec 19;6:39457. doi: 10.1038/srep39457

Figure 7. XEN cells contribute to the parietal endoderm of chimeric embryos.

Figure 7

(a–c) Pre-XEN cell line X-ICM-4 contributes to the PE of E6.5 and E7.5 embryos. GFP+ cells are derived from the injected pre-XEN cells. (a) Wholemount of E7.5 embryo. (b,c) Sections of the decidua of E6.5 embryos, showing the merged image of immunofluorescence for PDGFRa (red) and E-cadherin (blue), and fluorescence of DAPI (white) and GFP (green). (d–k) Post-XEN cell lines (X-E5.5-9, X-E6.5-Z0617-5, and X-E6.5-Z0617-2) contribute to the PE of E6.5 or E7.5 embryos. (d–f) Wholemount. GFP+ cells derived from X-E5.5-9 contribute to an E7.5 embryo. (g) Wholemount. GFP+ cells derived from X-E6.5-Z0617-5 contribute to an E7.5 embryo. (h,i) Wholemount. GFP+ cells derived from X-E6.5-Z0617-2 contribute to an E6.5 embryo. (j,k) Sections of the decidua of E7.5 embryos containing GFP+ cells derived from X-E6.5-Z0617-5, with immunofluorescence for PDGFRa (red) and E-cadherin (blue), fluorescence of DAPI (white) and GFP (green), and merge. The single GFP+ cell in (k) apposes but does not belong to the VE, as it is not immunoreactive for E-cadherin. (a–g) and (j,k) were imaged using a Zeiss LSM 710 confocal microscope, and (h–i) were imaged using a Nikon SMZ25 stereofluorescence microscope. In (a) and (e–g) the red counterstain is propidium iodide.