. 2016 Dec 20;6:39476. doi: 10.1038/srep39476

Table 1. ANOVA: Effects of the experimental temperatures and the site of origin and their interactions on the studied traits of C. fluminea.

Cellular process	Biomarker	Site	Temperature	Site × Temperature
	Gill tissue
OS	Mn-SOD	F_1,90 = 28.1 P < 0.001	F_2,90 = 36.2 P < 0.001	F_5,90 = 9.3 P < 0.001
OS	Cu,Zn-SOD	F_1,90 = 3.9 P = 0.05	F_2,90 = 13.4 P < 0.001	F_5,90 = 1.5 P = 0.202
OS	CAT	F_1,90 = 0.08 P = 0.772	F_2,90 = 24.1 P < 0.001	F_5,90 = 9.3 P < 0.001
OS	GSTμ	F_1,90 = 41.2 P < 0.001	F_2,90 = 21.6 P < 0.001	F_5,90 = 12.9 P < 0.001
OS	GSTM1	F_1,90 = 2.9 P = 0.095	F_2,90 = 13.1 P < 0.001	F_5,90 = 2.9 P = 0.018
OS	GSTS1	F_1,90 = 4.6 P = 0.035	F_2,90 = 0.09 P = 0.914	F_5,90 = 2.3 P = 0.051
OS	GPxA	F_1,90 = 9.8 P = 0.002	F_2,90 = 9.3 P < 0.001	F_5,90 = 3.0 P = 0.015
OS	TPX1	F_1,90 = 30.6 P < 0.001	F_2,90 = 1.3 P = 0.283	F_5,90 = 4.8 P = 0.001
OS	TXNLP	F_1,90 = 1.0 P = 0.313	F_2,90 = 2.0 P = 0.147	F_5,90 = 9.8 P < 0.001
OS	TXNRD1	F_1,90 = 0.89 P = 0.348	F_2,90 = 0.26 P = 0.774	F_5,90 = 9.2 P < 0.001
OS	MTs	F_1,90 = 0.72 P = 0.400	F_2,90 = 0.40 P = 0.670	F_5,90 = 4.3 P = 0.002
CH	HSP90	F_1,90 = 16.8 P < 0.001	F_2,90 = 33.9 P < 0.001	F_5,90 = 3.4 P = 0.008
CH	HSC70	F_1,90 = 4.6 P = 0.035	F_2,90 = 0.09 P = 0.914	F_5,90 = 2.3 P = 0.051
CH	HSP70*	F_1,90 = 18.2 P < 0.001	F_2,90 = 100.9 P < 0.001	F_5,90 = 14.8 P < 0.001
CH	HSP60*	F_1,90 = 35.6 P < 0.001	F_2,90 = 5.7 P = 0.005	F_5,90 = 22.7 P < 0.001
CH	HSP40	F_1,90 = 2.2 P = 0.143	F_2,90 = 11.4 P < 0.001	F_5,90 = 10.9 P < 0.001
CH	HSP22*	F_1,90 = 9.3 P = 0.003	F_2,90 = 1.1 P = 0.330	F_5,90 = 6.4 P < 0.001
	Foot muscle tissue
OS	MDA	F_1,54 = 55.0 P < 0.001	F_2,54 = 2.5 P = 0.096	F_5,54 = 4.9 P = 0.001
OS	4-HNE	F_1,54 = 53.2 P < 0.001	F_2,54 = 12.5 P < 0.001	F_5,54 = 9.4 P < 0.001
OS	PC	F_1,54 = 32.2 P < 0.001	F_2,54 = 1.0 P = 0.373	F_5,54 = 1.0 P = 0.438
PS	eIF-2α	F_1,37 = 10.5 P = 0.003	F_2,37 = 10.4 P = 0.001	F_5,37 = 4.7 P = 0.003
PS	p-eIF-2α	F_1,38 = 17.0 P < 0.001	F_2,38 = 22.6 P < 0.001	F_5,38 = 23.5 P < 0.001
EM	AMPK	F_1,39 = 146.3 P < 0.001	F_2,39 = 13.1 P < 0.001	F_5,39 = 7.7 P < 0.001
EM	HIF-1 α	F_1,34 = 18.0 P < 0.001	F_2,34 = 14.3 P < 0.001	F_5,34 = 28.8 P < 0.001
PD	Ubiquitin	F_1,54 = 0.04 P = 0.837	F_2,54 = 0.74 P = 0.484	F_5,54 = 4.7 P = 0.002
	Digestive gland
EM	PK	F_1,90 = 18.7 P < 0.0001	F_2,90 = 1.6 P = 0.1667	F_5,90 = 1.06 P = 0.4081
EM	PEPCK	F_1,90 = 0.002 P = 0.966	F_2,90 = 0.6 P = 0.7100	F_5,90 = 1.7 P = 0.0784
EM	LDH	F_1,90 = 8.212 P = 0.0048	F_2,90 = 0.1 P = 0.5494	F_5,90 = 1.5 P = 0.1574
EM	PK/PEPCK	F_1,90 = 5.7 P = 0.0187	F_2,90 = 1.4 P = 0.2164	F_5,90 = 2.0 P = 0.0332

Biomarkers for oxidative stress (OS), molecular chaperones (CH), protein synthesis (PS), protein degradation (PD) and energy metabolism (EM) were assessed. Significant effects (p < 0.05) are shown in bold. * - analyses were conducted on log-transformed data.