Figure 2. MEDI1912 dimer shows site-specific protection against hydrogen exchange.

HDX-MS identified peptide regions within MEDI1912 that have a lower rate of labelling under conditions that stabilise the antibody dimer (1 mg/mL) versus the monomer (0.1 mg/mL). Significant differences in labelling (p < 0.01) were observed in VHCDR1 (a), VHCDR2 (b) and VHCDR3 (c), yet there was no difference, for example, in a peptide within VH framework region 3). This supports the hypothesis that there is a specific interface for MEDI1912 self-association involving the variable domains. Data are corrected for back-exchange and represent mean ±1 SD.