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| Antibody | Mutations | HP-SEC monomer retention time (mins) |
|---|---|---|
| Reference IgG | N/A | 8.7 |
| MEDI-578 | N/A | 8.7 |
| MEDI1912 (WFL) | — | 15.0 |
| Mutant 1 (WFT) | L56T | 12.3 |
| Mutant 2 (WTL) | F31T | 10.3 |
| Mutant 3 (SFL) | W30S | 10.3 |
| Mutant 4 (SFT) | W30S, L56T | 9.7 |
| Mutant 5 (STL) | W30S, F31T | 9.0 |
| Mutant 6 (STT) | W30S, F31T, L56T | 8.9 |
