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. 2016 Dec 16;7:13783. doi: 10.1038/ncomms13783

Figure 5. The role of the LacI/GalR regulator Cphy2742 in galacturonic acid and pectin metabolism.

Figure 5

(a) Transcription of the LacI/GalR gene cphy2742 is up-regulated on galacturonic acid relative to other carbon sources. Bars shows average RNA-seq RPKM of duplicate cultures; error bars are one s.d. (bd) Upstream palindromes resembling cre operator sites found upstream of C. phytofermentans LacI/GalR genes and their orthologs from related genomes (b) cphy2742 (motif e=1.1 × 10−8), (c) cphy2467 (motif e=2.4 × 10−8) and (d) cphy1883 (motif e=8.9 × 10−2). (e) Twelve genes have both TSS (blue triangles) and putative Cphy2742 operators (red ovals) including genes for pectin lyases (green), galacturonic acid metabolism (purple), general carbon metabolism (yellow) and other or unknown (grey). The distance from the translation start is shown for each site.