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. 2016 Dec 15;7:13859. doi: 10.1038/ncomms13859

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Copyright © 2016, The Author(s)

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(a) Bright field microscopy: WB and Δcwp1-derived cysts/pseudocysts were tested for resistance to water (upper panels) and PBS (lower panels). WB cysts show refractile CWs; this optical effect is absent in Δcwp1 pseudocysts that appear damaged after exposure to water. Insets: individual cysts/pseudocysts. Scale bar: 25 μm. (b) tEM analysis: typical appearance of CWs (WB left panel); Δcwp1 pseudocysts lack a CW completely. Scale bar: 1 μm. Arrows indicate partially disassembled cytoskeletal elements. Insets scale bar: 0.2 μm. (c) AO/EB testing for cyst/pseudocyst viability highlights the fragility of cell culture-derived Δcwp1 pseudocysts compared with WB cysts. The y-axis indicates percentage.