Figure 5. The surface of Δcwp1 pseudocysts can capture soluble CWP1.
IFA analysis of Δcwp1 pseudocysts incubated with conditioned medium either from encysting (top, left panel) or non-encysting (top, right panel) WB cultures. Soluble CWP1 was captured on the surface of Δcwp1 pseudocysts only after incubation with conditioned medium from encysting WB cells but not trophozoites (left panels). WB cysts (control) react with the anti-CWP1 antibody (right panel). Δcwp1 or WB trophozoites are unable to capture soluble CWP1 from conditioned medium. Nuclei (blue) were stained with DAPI. DIC, differential interference contrast. Scale bar: 10 μm.