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. 2016 Dec 19;6:38574. doi: 10.1038/srep38574

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Copyright © 2016, The Author(s)

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(A) Western blotting results for expression of Kif2a were cropped gels. Oocytes were collected after culture for 0, 4, 8, 12 h, corresponding to the GV, Pro-M I, M I and M II stages, respectively. The molecular weight of Kif2a and β-actin were 110 kDa and 43 kDa, respectively. Each sample contained 200 oocytes. Full-length gels are presented in Supplementary Figure 1. The intensity of Kif2a/β-actin was assessed by grey level analysis. (B) Subcellular localization of Kif2a shown by immunofluorescent staining and confocal microscopy. Oocytes at various stages (GV, GVBD, M I,T Iand M II) were stained with antibody against Kif2a. Green, Kif2a; red, DNA; Magnifications of the boxed regions are shown. Bar = 20 μm.