Figure 1. Effect of the HMGA1 minigene on HMGA1 expression.

(a) HEK-293 cells were transiently transfected with 1 μg wild-type or mutant minigene construct, and endogenous HMGA1 mRNA and protein levels were measured 72 h later by qRT-PCR and Western blot (WB), respectively. Cropped blots are shown in the figure. Full-length WBs are presented in Supplementary Fig. S1. *P < 0.05 vs control (white bar). β-tubulin, control of protein loading. (b) Time course of HMGA1 mRNA abundance in HEK-293 cells, untreated (control, open square) or treated with wild-type (wt, solid circle) or mutant (mut, solid diamond) HMGA1 minigene. *P < 0.05 vs control. Data are mean ± standard error of the mean (s.e.m) of three independent experiments, each in triplicate.