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. 2016 Dec 19;6:39429. doi: 10.1038/srep39429

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Effect of hypoxia on VEGFA (gray bars) and HMGA1 (black bars) mRNA, in HepG2 cells preatreated or not with anti-HMGA1 siRNA, as measured by qRT-PCR. Data are means ± s.e.m of three independent experiments, each performed in triplicate. (b) ChIP of the VEGFA promoter gene in HepG2 and ARPE-19 cells, either untreated or pretreated with siRNA against HMGA1, both in normoxic and hypoxic conditions, using an anti-HMGA1 specific antibody (Ab). Representative assays are shown, together with qRT-PCR of ChIP-ed samples. Cropped gels are shown in the figures. Full-length ChIPs are presented in Supplementary Fig. S1.