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. 2016 Dec 20;6:39559. doi: 10.1038/srep39559

Figure 1. Effects of HDM on the TSLP isoforms expression and the barrier integrity of 16HBE cells.

Figure 1

(a) The expression of E-cadherin and β-catenin was assessed using western blotting in 16HBE cells stimulated with HDM (400 U) for the indicated times. (b) Western blotting was used to detect lfTSLP protein in the cells and the culture medium of 16HBE cells stimulated with HDM for the indicated times. (c) Western blotting analysis of E-cadherin, β-catenin, and lfTSLP proteins in 16HBE cells stimulated with different concentrations of HDM for 6 h. (d) RT-PCR assay of the long and short splice forms of TSLP mRNA in 16HBE cells stimulated with HDM (400 U) for the indicated times. (e) RT-PCR assay of the long and short splice forms of TSLP mRNA in 16HBE cells stimulated with the indicated doses of HDM for 4 h. (f) Transepithelial electrical resistance (TER) was measured to determine epithelial barrier integrity after the cells were treated with HDM (400 U) for the indicated times. (g) The FITC-Dx permeability was measured to determine epithelial barrier integrity after the cells were treated with HDM (400 U) for the indicated times. Data are presented as the mean ± SD; n = 3–4.*P < 0.05 vs. the control group.