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. 2016 Dec 20;6:39559. doi: 10.1038/srep39559

Figure 3. HDM-induced lfTSLP upregulation is mediated by the MAPK signaling cascade in 16HBE cells.

Figure 3

(a,b) Activation of three MAPK pathway proteins, (phosphorylated [p]-MAPK/total [t]-MAPK], p-ERK:t-ERK, p-p38:t-p38, and p-c-Jun N-terminal kinase (JNK):t-JNK was measured by western blotting and densitometric analyses in 16HBE cells stimulated with HDM (400 U) for the indicated times. (c) 16HBE cells were respectively treated with culture medium (control group), U0126 (10 μM), or HDM (400U) with or without U0126 (10 μM) for 1 h prior to stimulation with HDM. The expression of p-ERK, t-ERK, and lfTSLP proteins was evaluated using western blotting analysis in the treated cells. (d) 16HBE cells were respectively treated with culture medium (control group), SB203580 (10 μM), or HDM (400 U) with or without SB203580 (10 μM) for 1 h prior to stimulation with HDM. The expression of p-p38, t-P38, and lfTSLP protein was evaluated using western blotting analysis in the treated cells. Data are presented as the mean ± SD; n = 3–4. *P < 0.05 vs. the control group; #P < 0.05 vs. the HDM group.