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. 2016 Dec 14;7:13627. doi: 10.1038/ncomms13627

Figure 5. α-gH mAbs bind to multiple glycoprotein complexes.

Figure 5

(a) Lysates from metabolically labelled TB40/E-infected MRC5 cells were exposed to an isotype control mAb recognizing GFP, or mAb 10C10 and 5C3. Recovered immune complexes were split and treated with PNGase F, then resolved by non-reducing SDS–PAGE. Arrows denote the identity of glycosylated protein complexes and asterisks denote the identity of de-glycgosylated protein complexes. (b) Lysates from TB40/E-infected MRC5 cells were exposed to a GFP mAb, 10C10 or 5C3. Immune complexes were resolved by non-reducing SDS–PAGE followed by immunoblot for gH (lanes 1–3), gL (lanes 4–6) and UL128 (lanes 7–9). Lines denote the glycoprotein complexes. (c) Lysates from U373 cells stably expressing gH/gL/UL128 (U373gH/gL/UL128) and gH/gL/gO-HA (U373gH/gL/gO-HA) were exposed to GFP, 10C10 or 5C3 mAbs and the recovered immune complexes were resolved by SDS–PAGE and subjected to immunoblot for gH (lanes 1–6), HA (lanes 7–12), gL (lanes 13–18) and UL128 (lanes 19–24).