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. 2016 Dec 19;6:39047. doi: 10.1038/srep39047

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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A–A3: gastrula stage; B-B3: embryonic stage; C-C3: metanauplius stage; (A,B and C) represent single-labeling with anti-CDC20; A1-C1: single-labeling with anti-CDH1; A2-C2 represent the image overlay of dual-labeled samples. A3–C3 represent the control group.