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. 2016 Dec 20;6:39349. doi: 10.1038/srep39349

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Copyright © 2016, The Author(s)

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1. Mix aqueous phase together, comprising either A: MFS protein reconstituted into LUVs or B: cell-free reaction with added empty liposomes and template DNA. Prepare second droplet containing substrate and empty liposomes. 2. Submerge sub-microlitre droplet of the aqueous phase in hexadecane solvent bath and incubate to allow monolayer formation around each individual droplet. 3. Manipulate two droplets together so that a bilayer interface is formed between them. 4. Use microscopy to take images of the DIB and to track the transport of fluorescent substrates.