Figure 2. QS-21 induces rapid changes in gene expression in draining lymph node.

(A,B) Monocyte (CD11b⁺ Ly6C⁺ Ly6G⁻), neutrophil (SSC^hi CD11b⁺ Ly6C^int Ly6G⁺), eosinophil (SSC^hi CD11b⁺ Ly6C⁺ Ly6G⁻ SiglecF⁺) and dendritic cell (CD11c⁺ MHCII⁺) recruitment was assessed by flow cytometry 24 h post immunisation in the muscle (A) and draining lymph node (B). Each point represents a pool of 2 (DLN) or 3 (muscle) mice and the horizontal bar is the geometric mean. (C) Heatmap representation of mRNA expression (Fold change over PBS) of the top upregulated genes (Fold Change over PBS >10 in either the injection site or draining lymph node) measured by microarray analysis. (D,E) Fold change over PBS of the top 50 upregulated genes at 2, 4 or 6 hours in the draining lymph node (D) or injection site (E). The top upregulated genes in the draining lymph node (Cxcl1, il6, il1b) are represented on each chart. (F) InnateDB analysis of pathway over-representation of significantly upregulated genes (p-value < 0.05 and fold change >3) using the hypergeometric algorithm and Benjamini Hochberg correction for p-values.