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. 2016 Dec 20;6:39475. doi: 10.1038/srep39475

Figure 7. QS-21-elicited neutrophil and dendritic cell recruitment, cellular CD4/CD8 T-cell responses and antibody production are dependent on MyD88.

Figure 7

(A) Innate cell recruitment in the iliac DLN 24 h post QS-21 injection. Absolute numbers of monocytes (Lin− CD11b+ Ly6C+ Ly6G−), neutrophils (SSChi Lin− CD11b+ Ly6Cint Ly6G+) and dendritic cells (Lin− CD11c+ MHCII+) per DLN were assessed by flow cytometry (PBS: n = 6, QS-21: n = 7–8). (B–D) WT and MyD88 KO mice were immunised as in 1 A. At day 21, median cytokine production (B) and polyfunctionality (C) of HBs-specific CD4 were evaluated by intracellular staining (Ag: n = 3, QS-21: n = 14). (D) Cytokine production of HBs-specific splenic CD8 T cells and frequency of OVA-specific circulating CD8 T cells assessed by flow cytometry (Ag: n = 3–6, QS-21: n = 14–21). (E) Anti-HBs IgG1 and IgG2c titres in the serum at day 21 were measured by ELISA (Ag: n = 6, QS-21: n = 21). Each point represents one mouse and the horizontal bar represents the geometric mean. Statistical significance was determined by a non-parametric Mann-Whitney test. The data is representative of 2 independent experiments.