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. 2016 Dec 20;6:39311. doi: 10.1038/srep39311

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Schematics of the Foxg1 locus including saRNA positions and orientations as well as the diagnostic qRTPCR amplicon. (B–D) Lentiviral reagents and protocols employed for this screening. (E,F) Foxg1-mRNA levels in proliferating neocortical precursors and their differentiating derivatives, manipulated as in (C) and (D), respectively. Values double normalized, against Gapdh and control (NC). E, embryonic day. DIV, days in vitro. Bars represent sem’s. n = number of biological replicates. p-values were calculated by the t-Student algoritm (one-tail, unpaired). All results with p < 0.05 further passed Benjamini-Hochberg filtering, with FDR < 1/m.