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. 2016 Dec 20;6:39311. doi: 10.1038/srep39311

Figure 2. Histogenetic outcome of Foxg1-RNAa.

Figure 2

(A,B) Protocols and lentiviral reagents employed for this assay. (C) Quantification of cells immunopositive for the neuron-specific Tubβ3 marker, in cultures of neocortical precursors expressing Foxg1-saRNAs. (D) Examples of Tubβ3+ immuno-fluorescences referred to in (C). E, embryonic day. DIV, days in vitro. Bars represent sem’s. n = number of biological replicates. Statistical significance of results evaluated by t-Student assay (one-tail, unpaired). Absolute average frequency of Tubβ3+ cells in NC samples was (27.25 ± 0.16)%.