Table 1. Transition temperatures and statistics for thermal denaturation.
| Protein | Transition temperature/K | P value for Student’s t test |
|---|---|---|
| FKBP12 | 332.1 ± 0.1 | — |
| Ub-FKBP12 | 316.5 ± 0.2 | 1.5 × 10−7 |
| Ub6-FKBP12 | 314.8 ± 0.3 | 5.5 × 10−7; 7.8 × 10−3* |
| FKBP12 + Ub | 334.9 ± 0.2 | 1.5 × 10−4 |
| FKBP12K36C | 334.5 ± 0.3 | — |
| Ub-ss-FKBP12C36 | 335.1 ± 0.4 | 2.7 × 10−1 |
| FKBP12K48C | 327.7 ± 0.3 | — |
| Ub-ss-FKBP12C48 | 320.9 ± 0.2 | 6.4 × 10−5 |
| FKBP12K53C | 325.9 ± 0.3 | — |
| Ub-ss-FKBP12C53 | 323.6 ± 0.0 | 2.1 × 10−3 |
| FABP4 | 336.1 ± 0.2 | — |
| Ub-FABP4 | 328.8 ± 0.0 | 4.8 × 10−6 |
| Ub6-FABP4 | 327.4 ± 0.1 | 3.9 × 10−6; 3.6 × 10−4* |
| FABP4 + Ub | 335.8 ± 0.3 | 4.4 × 10−1 |
| FABP4K32C | 324.6 ± 0.4 | — |
| Ub-ss-FABP4C32 | 324.3 ± 0.2 | 4.3 × 10−1 |
| FABP4K80C | 324.7 ± 0.1 | — |
| Ub-ss-FABP4C80 | 324.4 ± 0.1 | 4.4 × 10−2 |
| FABP4K121C | 326.5 ± 0.1 | — |
| Ub-ss-FABP4C121 | 326.2 ± 0.1 | 9.7 × 10−2 |
The superscripts show the point mutations or the site of disulfide bridge formation. The asterisks indicate the P values in Student’s t test between the transitions of mono- and hexaubiquitylated proteins.