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. 2016 Dec 16;7:13846. doi: 10.1038/ncomms13846

Figure 2. ORP1 is expressed in the oocyst capsule and localization is independent of ORP2.

Figure 2

(a) Localization of ORP1 using an antibody directed against a peptide of the HFD in ookinetes, oocysts at 10 days p.b.f. and sporozoites. Scale bars, 2.5, 15 and 10 μm, respectively. The ookinete was also labelled with an antibody directed against the P28 surface protein (red). Nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI; blue). BF, bright field image. (b) Schematic depiction of a construct encoding a GFP fusion of the N-terminal 365 aa fragment of ORP1. (c) Localization of ORP::GFP in ookinetes, oocysts at 6 days p.b.f. and sporozoites using immunolabelling. Of note is the localization of ORP1::GFP in proximity to Cap380 in the oocyst periphery. Scale bars, 2.5, 10 and 5 μm, respectively. (d) ORP1 is localized to the oocyst capsule revealed by double labelling with antibodies recognizing ORP1 and CSP, at day 7 bpf (scale bars, 10 μm (top row) and 2.5 μm (bottom row)). (e) Immunolabelling of an orp2(−) mutant oocyst reveals localization of ORP1 (red) at the oocyst periphery. Scale bar, 15 μm.