Table 1. Midgut and salivary gland sporozoites.
Experiment | Parasite | Midgut sporozoites | Salivary gland sporozoites | Number of mosquitoes dissected |
---|---|---|---|---|
#1 | WT | 0 | 4,050 | 61 |
orp1(−) cl1 | 4,600 | 0 | 59 | |
orp2(−) cl1 | 5,900 | 0 | 46 | |
hfd− cl 1 | 3,450 | 0 | 45 | |
#2 | WT | 0 | 4,100 | 51 |
orp1(−) cl2 | 4,400 | 0 | 43 | |
orp2(−) cl2 | 4,050 | 0 | 49 | |
hfd− cl 2 | 2,700 | 0 | 45 | |
#3 | WT | 22,917 | 25,900 | 240 |
orp1(−) cl2 | 24,885 | 0 | 217 | |
orp2(−) cl2 | 16,188 | 0 | 244 |
In experiment 1 and 2, midguts and salivary glands were dissected at day 21 p.b.f. In each experiment, the data are pooled from two experiments of each clone. The complete data set is presented in Supplementary Table 1. In experiment 3, midgut and salivary glands were dissected from the same mosquito at day 18 p.f.b. In all experiments, the organs were homogenized and sporozoites counted under the microscope.