Table 2. Infectivity of sporozoites in mice.
| Experiment | Parasite | Pre-patent* | Number of infected mice |
|---|---|---|---|
| #1 Mosquito bite | WT | 3.0 | 3/3 |
| orp1(−)cl1 | 0 | 0/3 | |
| #2 Mosquito bite | WT | 3.0 | 3/3 |
| orp2(−)cl1 | 0 | 0/3 | |
| #3 Mosquito bite | WT | 3.0 | 3/3 |
| orp1hfd− cl1 | 0 | 0/3 | |
| #4 Intravenous injection | WT | 6.5 | 8/8 |
| orp1(−)cl1 | 0 | 0/8 | |
| orp2(−)cl1 | 0 | 0/8 | |
| #5 Intravenous injection | WT | 5.5 | 8/8 |
| orp1(−)cl2 | 0 | 0/8 | |
| orp2(−)cl2 | 0 | 0/8 |
Experiment 1 to 3: 20 infected mosquitoes were allowed to feed on naive C57BL/6 mice. Experiment 4 and 5: 5 × 105 sporozoites isolated from the midguts of infected mosquitoes 18 days p.b.f. were injected intravenously in the tail vein of C57BL/6 mice.
*Pre-patent: the time between sporozoite inoculation and the appearance of parasites in the blood (days).