Figure 2. Association between NKG2D genotypes and NKG2D-mediated effector functions.

(A) The MICA/B expression on the surface of OUN1 cells with (blue histogram) or without VPA treatment (orange histogram), as assessed by flow cytometry, (red histogram; isotype stained cells). The representative results of three independent experiments are shown. (B) The cytotoxicity of HNK1/HNK (n = 6) and LNK/LNK (n = 13) NK cells against OUN-1 cells with or without VPA treatment (expressing NKG2D-L) in the presence or absence of a blocking anti-NKG2D mAb, as determined by a ⁵¹Cr release assay. (C) The expression of ULBP3 in uninfected (orange histogram) or CMV infected (blue histogram) HEL cells as assessed by flow cytometry (D) Cytotoxicity of HNK1/HNK (n = 6) and LNK/LNK (n = 13) NK cells against CMV-infected human fibroblasts in the presence or absence of a blocking anti-NKG2D mAb. (E) IFN-γ secreted by PBMC from individuals with the HNK1/HNK genotype (n = 3) and LNK/LNK (n = 7) cultured with or without NKG2D-Ls and assessed by an ELISA assay.