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. 2016 Dec 20;6:39231. doi: 10.1038/srep39231

Figure 5. Effects of rs1049174 variant on NK cells susceptibility to transforming growth factor.

Figure 5

PBMCs from individuals with HNK/HNK (3) or LNK/LNK (3) genotype were cultured for four days in IL-2 containing medium in the presence of either recombinant MICA (NKG2D-L) or TGFβ1 (10 ng/ml) and the expression of NKG2D receptor on NK cells and CD8+ T cells was assessed by flow cytometry. (A) The gating strategy used to assess NKG2D expression in NK cells and CD8+ T cells. (B) A representative histogram figure showing NKG2D expression in control (dark histogram), rMICA treated, (blue histogram) or TGFβ1 treated (red histogram) NK cells is shown in the upper panel. The bottom panel shows summarized results from different individuals with HNK1/HNK (n = 3) and LNK/LNK (n = 3) and the data represent the NKG2D intensity (% MFI) in treated cells compared with that in the control cells. (C) A representative histogram figure showing NKG2D expression in control (dark histogram), rMICA treated, (blue histogram) or TGFβ1 treated (red histogram) CD8+ T cells is shown in the upper panel. The bottom panel shows summarized results from different individuals with HNK1/HNK (n = 3) and LNK/LNK (n = 3) and the data represent the NKG2D intensity (% MFI) in treated cells compared with that in the control cells.