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Granulocytes are isolated from peripheral blood samples donated by asthmatic (n=26) and non-asthmatic (n=15) volunteers and analyzed with flow cytometry. Flow diagram shows the gating strategies for IL-17RB+ granulocytes, T2M (CD11B+CD16+CD177+IL-17RB+ granulocytes), and IL-17RB+ eosinophils (IL-17RB+CD16-granulocytes). Granulocytes were selected first and then subsequently gated for the subgroups. The bottom panel shows correspondent isotype control for each cell type.
