Table 4.
Inferred HLA allele restriction analysis performed using the RATE analysis tool. A- Allele, R- Responder, RF- relative frequency, OR-odds ratio. P<0.05 is considered significant.
| Number of donors | Percentage of of donors | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Antigen | Start | Peptide | Allele(s) of inferred restriction | A+R+ | A− R+ |
A+R− | A− R− |
A+R+ | A− R+ |
A+R− | A− R− |
No. of donors |
RF | OR | p- value |
| Cry j 1 | 233 | SMKVTVAFNQFGPNCG | DPB1*05:01,DRB5*01:01,DRB1*04:01 | 9 | 0 | 7 | 16 | 28% | 0% | 22% | 50% | 32 | 2.0 | inf | 0.001 |
| Cry j 2 | 121 | DGIIAAYQNPASWKNN | DRB1*15:02,DRB5*01:01,DRB5*01:02,DRB5*02:02 | 8 | 2 | 3 | 19 | 25% | 6% | 9% | 59% | 32 | 2.3 | 25.3 | 0.001 |
| Cry j 2 | 113 | QPHFTFKVDGIIAAYQ | DPB1*02:01,DPB1*09:01,DRB1*15:02,DPB1*01:01 | 7 | 0 | 8 | 17 | 22% | 0% | 25% | 53% | 32 | 2.1 | inf | 0.002 |
| Cry j 2 | 393 | IKLSDISLKLTSGKIA | DPB1*02:01,DRB1*15:02,DRB5*01:02 | 6 | 0 | 6 | 20 | 19% | 0% | 19% | 63% | 32 | 2.7 | inf | 0.001 |
| Cry j 2 | 217 | VKIIGISITAPRDSPN | DRB1*15:02,DRB1*04:03,DRB5*01:01,DRB5*01:02,DRB1*14:01 | 6 | 1 | 5 | 20 | 19% | 3% | 16% | 63% | 32 | 2.5 | 24.0 | 0.003 |
| Cry j 2 | 401 | KLTSGKIASCLNDNAN | DRB1*04:04,DQB1*03:03,DRB1*15:02,DRB1*04:01 | 5 | 1 | 4 | 22 | 16% | 3% | 13% | 69% | 32 | 3.0 | 27.5 | 0.003 |
| Cry j 2 | 129 | NPASWKNNRIWLQFAK | DRB1*15:01,DPB1*18:01,DPB1*11:01,DRB1*15:02 | 5 | 1 | 4 | 22 | 16% | 3% | 13% | 69% | 32 | 3.0 | 27.5 | 0.003 |
| Cry j 2 | 89 | PSAMLLVPGSKKFVVN | DRB1*13:03,DRB1*15:02,DRB1*03:02,DRB5*01:02 | 5 | 1 | 1 | 25 | 16% | 3% | 3% | 78% | 32 | 4.4 | 125.0 | 0.000 |
| Cry j 1 | 321 | QDVFYNGAYFVSSGKY | DPB1*18:01,DPB1*11:01,DPB1*05:01,DRB3*01:01 | 5 | 0 | 6 | 21 | 16% | 0% | 19% | 66% | 32 | 2.9 | inf | 0.002 |
| Cry j 2 | 137 | RIWLQFAKLTGFTLMG | DPB1*18:01,DPB1*11:01,DRB1*15:02,DRB5*01:02,DPB1*09:01,DPB1*03:01 | 5 | 2 | 2 | 23 | 16% | 6% | 6% | 72% | 32 | 3.3 | 28.8 | 0.002 |
| Cry j 1 | 281 | TILSEGNSFTAPNESY | DRB1*04:05,DRB1*15:02,DRB1*04:01 | 5 | 1 | 4 | 22 | 16% | 3% | 13% | 69% | 32 | 3.0 | 27.5 | 0.003 |
| Cry j 1 | 129 | VFIKRVSNVIIHGLYL | DRB1*14:04,DRB1*04:04,DRB1*15:02,DRB5*01:02 | 5 | 3 | 1 | 23 | 16% | 9% | 3% | 72% | 32 | 3.3 | 38.3 | 0.002 |
| Cry j IFR |
121 | AVEPMKSMFDLKIKLR | DRB1*16:02,DRB5*02:02,DRB1*13:01,DRB1*03:01 | 4 | 0 | 2 | 26 | 13% | 0% | 6% | 81% | 32 | 5.3 | inf | 0.000 |
| Cry j 2 | 81 | AWQAACKNPSAMLLVP | DRB1*15:02,DRB1*13:03,DRB1*03:01 | 4 | 0 | 5 | 23 | 13% | 0% | 16% | 72% | 32 | 3.6 | inf | 0.004 |
| Cry j 1 | 257 | GLVHVANNNYDPWTIY | DRB1*04:05,DRB1*15:02,DQB1*05:03 | 4 | 0 | 5 | 23 | 13% | 0% | 16% | 72% | 32 | 3.6 | inf | 0.004 |
| Cry j 1 | 353 | GNATPQLTKNAGVLTC | DRB3*03:01,DRB1*15:02,DRB1*13:03,DRB1*13:02 | 4 | 4 | 2 | 22 | 13% | 13% | 6% | 69% | 32 | 2.7 | 11.0 | 0.023 |
| Cry j 1 | 89 | LWIIFSGNMNIKLKMP | DPB1*11:01,DRB1*15:02,DPB1*09:01,DRB1*04:03,DRB5*01:02,DRB1*14:01 | 4 | 2 | 1 | 25 | 13% | 6% | 3% | 78% | 32 | 4.3 | 50.0 | 0.002 |
| Cry j 2 | 361 | QIQDVTYKNIRGTSAT | DRB1*15:01,DRB1*15:02,DRB5*01:02,DRB1*04:01 | 4 | 0 | 6 | 22 | 13% | 0% | 19% | 69% | 32 | 3.2 | inf | 0.006 |
| Cry j 1 | 209 | SNNLFFNHHKVMLLGH | DRB1*08:01,DRB1*04:01,DRB1*03:01 | 4 | 1 | 3 | 24 | 13% | 3% | 9% | 75% | 32 | 3.7 | 32.0 | 0.004 |
| Cry j 1 | 313 | SNWVWQSTQDVFYNGA | DRB1*09:01,DRB1*15:02,DRB1*04:01,DPB1*09:01 | 4 | 0 | 5 | 23 | 13% | 0% | 16% | 72% | 32 | 3.6 | inf | 0.004 |