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. 2016 Dec 20;6:39270. doi: 10.1038/srep39270

Table 3. Strains and plasmids used in this study.

Strain or plasmid Characteristics Source or reference
Hydrogenophaga intermedia
 QY7-2 Wild type; MDE-degrading strain; Smr This study
 Tn5 mutants QY7-2; mdeB::Tn5 Smr Kmr This study
E. coli strains
 DH5α F—; recA1; endA1; thi-1; supE44; relA1; deoR; Δ (lacZYA-argF)U169; Φ80dlacZΔM15 Takara
BL21(DE3) F—; ompT; hsdSB(rB— mB—); dcm(DE3); gal; λ(DE3) Invitrogen
 SM10λpir thi thr leu tonA lacY supE recA RP4-2-Tc::Mu λ::pir Lab stock
 HB101 (pRK2013) Conjugation helper strain Lab stock
Plasmids
 pSC123 Mariner transposon; Cmr Kmr Lab stock
 pMD19-T TA clone vector; Apr TaKaRa
 pET-29a(+) Expression vector; Kmr Novagen
 pETA pET-29a(+) carrying mdeA; Kmr This study
 pETB pET-29a(+) carrying mdeB; Kmr This study
 pETC pET-29a(+) carrying mdeC; Kmr This study
 pETD pET-29a(+) carrying mdeD; Kmr This study