Table 9.

The sequences of primers and cycling conditions.

Gene	Primers	DNA sequence	cycling condition
IL-18	sense	5′-AGGAATAAAGATGGCTGCTGAAC-3′	95°C 50s, 60°C 1min, 72°C 45s, for 32cycles
	antisense	5′-GCTCACCACAACCTCTACCTCC-3′
IL-6	sense	5′-CTACATTTGCCGAAGAGCCCT-3′	95°C 30s,58°C 30s, 72°C 1min, for 35 cycles
	antisense	5′-CTCGACGGCATCTCAGCC-3′
COX-2	sense	5′-TTCAAATGAGATTGTGGGAAAATTGCT-3′	94°C 56s, 50°C 90s,72°C 2min, for 30 cycles
	antisense	5′-AGATCATCTCTGCCTGAGTATCTT-3′
MMP-1	sense	5′-ACGGATACCCCAAGGACATCT-3′	95°C 30s,55°C 30s,72°C 1 min, for 35 cycles
	antisense	5′-CTCAGAAAGAGCAGCATCGATATG-3′
β-actin	sense	5′-GGCCATCTCTTGCTCGAAGT-3′	94°C 30s, 56°C 30s, 72°C 1min, for 30 cycles
	antisense	5′-GCCCAGAGCAAGAGAGGCAT-3′