Fig 6. EndoU-mediated MAVS reduction exhibited impaired IFN signaling.

(A-C) MARC-145 cells were co-transfected with pIFN-β-luc (0.5 μg) (A), or p4xIRF3-luc (0.5 μg) (B), or p(PRDII)₂-luc (0.5 μg) (C), pRL-TK (0.05ug), and wild-type nsp11 (0.5 μg) or individual nsp11 EndoU mutant plasmids (0.5 μg/plasmid). At 24 h post transfection, cells were treated with poly(I:C) (0.5 μg/ml) for 16 h. Cells were lysed and examined for luciferase activity. The experiments were conducted in duplicate and repeated three times. Each sample expressing nsp11 was compared with poly(I:C) stimulated samples (*, P<0.05; **, P<0.01 by one-way ANOVA and bonferroni multiple comparison). (D) HeLa cells were co-transfected with empty vector control, or nsp11, or K3779A, and at 24 h, treated with 20 ng/ml of TNF-α for indicated time, followed by Western blot.