Figure 1. Memory T_H1 responses in μMT-/- mice versus wt mice.

We immunized wt and μMT-/- mice with ID93/GLA-SE three times, at 3-week intervals, for the analysis of ID93-specific immune responses. CD154 expression and IFN-γ, TNF, and IL-2 production from CD4+ T cells was measured by flow cytometry. We assessed ID93-specific recall immune single (A and B) and polyfunctional (C and D) responses following ID93 stimulation of splenocytes (A and C) or dLN cells (B and D) with 10 μg ml^–1 ID93 6 weeks after the final immunization. The gating strategy is shown in Supp. Inf. Fig. 2. Graphs show mean values ± SEM for each group. The p values were determined by two-way ANOVA with Bonferroni correction for multiple comparisons (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001). Data are representative of three independent experiments with three mice per group.