Figure 5. MPEC generation in μMT-/- mice following adoptive transfer of B cells from either wt or MHCII-/- mice.

We adoptively transferred B cells from wt or MHCII-/- mice into μMT-/- mice. Three days later, we immunized wt, μMT-/- or μMT-/- adoptively transferred mice once with ID93/GLA-SE for the analysis of ID93-specific immune responses. Peptide-MHCII tetramer staining was used to identify different ID93-specific T cell subsets. Representative flow cytometry plots and graphs are shown for the percentage of CD4 T cells that bind the tetramer (A), the percentage of tetramer-binding CD4 T cells that are PD1⁺/KLRG1^- (B) and the percentage of tetramer-binding CD4 T cells that are T-bet^Lo/Ly6C^Lo (C). The gating strategy is shown in Supp. Inf. Fig. 5. Graphs show mean values ± SEM for each group. The p values were determined by one-way ANOVA with Bonferroni correction for multiple comparisons (#p<0.001compared to all other groups). Data are representative of two independent experiments with five mice per group.