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. 2016 May 17;7(27):41053–41066. doi: 10.18632/oncotarget.9419

Figure 3. Desialylated MoDCs show higher levels of maturation and expression of pro-inflammatory cytokines.

Figure 3

A. Desialylated MoDCs show a higher maturation phenotype than fully sialylated MoDCs. The expression of several maturation markers was evaluated by flow cytometry. MoDCs were first treated with sialidase (Sia) for 1h at 37°C and then loaded (A, right panel) or not (A, left panel) with MCF-7 lysates (TL), for 3h at 37°C, as described in Materials and methods. Graph values represent the mean fluorescence intensity (MFI) (average ± SEM) of at least 3 independent assays. Statistically significant differences were calculated using t-test (*P < 0.05, **P < 0.01 and ***P < 0.0001) and refers to the difference between untreated and sialidase treated conditions. B. Sialidase treatment of MoDCs elicits IL-12 secretion. The cytokines secreted by MoDCs following sialidase treatment were measured by ELISA. Data represent the cytokine concentration (pg/mL) (average ± SEM) of 6 independent experiments. Statistically significant differences were calculated using t-test (*P < 0.05 and **P < 0.01) and refers to the difference between untreated and sialidase treated conditions.