Figure 7. Sialidase treatment of human MoDCs improves antigen cross-presentation via MHC-I to CD8⁺ T cells.

Gp100-specific CD8⁺ T cells were co-cultured overnight with gp100-loaded MoDCs, that have been previously treated or not with sialidase (MoDC^sia+gp100 and MoDC^gp100, respectively). To evaluate the activation of CD8⁺ T cells, the secretion of IFN-γ cytokine was measured by ELISA. A. Short gp100 peptides or B. different concentrations of the long gp100 peptide were used to load MoDCs. Graph values are normalized to the condition of MoDC^gp100 with the short peptide. Data refers to the IFN-γ secretion (ng/mL) (average ± SEM) of 3 independent experiments. Statistical significance (*P < 0.05) refers to the difference between fully sialylated and desialylated gp100-loaded MoDCs.