Figure 4. YLL545 inhibits angiogenesis in vivo.

A. 12-hpf zebrafish embryos were incubated with different concentrations of YLL545 or sorafenib. At 36 hpf, fluorescent assays were performed to visualize the growth of ISVs and DLAVs, which were manually counted. **P < 0.01 and ***P < 0.001 vs respective control in Student's t-test. Scale bars, 100 μm. B. At the indicated time points, fluorescent assays were performed to visualize the growth of caudal vessels, which were manually counted. *P < 0.05 and **P < 0.01 vs respective control in Student's t-test. Scale bars, 300 μm. C. Matrigel containing 80 mg/kg YLL545 or 80 mg/kg sorafenib was subcutaneously injected into mice, and the plugs were surgically removed after 10 days. Expression of CD31 in plugs from YLL545- or sorafenib-treated mice was examined by immunofluorescent staining. Scale bars, 100 μm.