Figure 5. Silencing GLIS3 results in the abrogation of melanoma invasion in vitro.

(A) GLIS3 expression was evaluated in ten mesenchymal- and epithelial-like melanoma cells by qRT-PCR. Bars indicate mean +/− SEM (t-test, **p < .05). (B) 72 h after transfection of six melanoma cell lines with either 10 nM control siRNA or GLIS3 specific siRNA GLIS3 qRT-PCR was performed (t-test, **p < .005, ***p < .0005). (C–D) The in vitro invasive ability of these cells lines was tested using a Matrigel assay. (C) Representative images of invasive cells were taken (scale bar = 100 μm). (D) Average intensities of invasive cells were calculated in K counts mm² using Odyssey Software. Bars indicate mean +/− SEM of three independent experiments in triplicate. Data was combined with data from same cell lines in Figure 3 and Figure 4 and analysed using ANOVA, with post-hoc Tukey test to identify treatments significantly different from control (**p < .005, ***p < .0005).