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. 2016 May 18;7(27):41445–41459. doi: 10.18632/oncotarget.9441

Figure 2. Inhibition of ROCK2 but not ROCK1 sensitized HCC cells to chemotherapy.

Figure 2

(A and B) Western blot analysis was used to confirm the successful knockdown of ROCK1/2 by both sh- and si-RNA. The amount of cell death 48 hours after treatment with cisplatin was quantified. Only knockdown of ROCK2 enhanced cisplatin-induced cell death. (C) Knockdown of the RhoA protein by siRNA approach was confirmed by Western blot analysis. The amount of cell death 48 hours after treatment with cisplatin was quantified. RhoA knockdown sensitized HCC cells to cisplatin treatment. (D) Knockdown of RhoA and ROCK2 rescued the enhanced chemoresistance induced by RhoE knockdown in HCC cells. In all panels, 3 experiments are represented. P-value was calculated using Student's t test.