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. 2016 May 6;7(27):41505–41526. doi: 10.18632/oncotarget.9214

Figure 3. Regulation mechanisms of lincRNA-p21 expression.

Figure 3

A. p53 enrichment at the human lincRNA-p21 promoter in GSCs and non-GSCs glioma cells as quantified by Real-time RT-PCR. The binding efficiency of p53 and lincRNA-p21 promoter in GSCs showed no significant difference compared to the corresponding non-GSCs glioma cells. B. Detection of HuR expression levels in GSCs and non-GSCs glioma cells by Western blot. C. Real-time RT-PCR analysis of let-7b expression levels in GSCs and non-GSCs glioma cells. *P<0.01 vs NHA. D. Transcriptional activation of HuR through PI3K/AKT pathway in both GSCs and non-GSCs glioma cells. E. HuR protein expression levels were reduced in both GSCs and non-GSCs glioma cells after AKT and mTOR-siRNA transfection. F. Real-time RT-PCR analysis of HuR mRNA levels in both GSCs and non-GSCs glioma cells after AKT and mTOR-siRNA transfection. *P<0.01 vs nc-siRNA.