Skip to main content
. 2016 May 12;7(27):41637–41649. doi: 10.18632/oncotarget.9317

Figure 2. Cisplatin resistant cells depend on glutamine for oxidative phosphorylation.

Figure 2

A. Ovarian cancer cell lines were cultured in complete media, glutamine free or glutamine free containing α-KG (4 mM). B. Added GLN restores cell viability of CP70 and CP10 cells but not A2780 or OV81.2 cells. Cell viability was determined using the Vi-CELL trypan blue exclusion counter. Experiments were performed in triplicate and repeated 3 times. Viability is expressed relative to the cell line grown in complete media. Graphs represent mean (boxes) and SD (bars; n=9). C. A2780 or CP70 D. OV81.2 or CP10 cells were treated with DMSO or BPTES (50uM) for 24 hr before measuring oxygen consumption rate (OCR). OCR is increased in resistant cell lines compared to the sensitive cell lines. The decrease using BPTES (50uM) confirms the glutamine dependency of cisplatin resistant CP70 and CP10 cells. Experiments were carried out in triplicates and repeated 3 times. Graphs represent mean (dots) and SD (bars; n=9). E. GLS over expression in the sensitive cell line OV81.2 results in increased OCR compared to OV81.2 control (p<0.01). CP10 shGLS transduced cells have reduced OCR compared to CP10 shRNA control (p<0.01).