Skip to main content
. 2016 May 12;7(27):41637–41649. doi: 10.18632/oncotarget.9317

Figure 3. c-Myc regulates glutamine metabolism in cisplatin resistant cells.

Figure 3

A. Western blot showing increased c-Myc expression in the resistant cell lines compared to the sensitive cell lines as well as increasing c-Myc expression in A2780 and OV81.2 cells after 24 and 48hr of cisplatin (2uM) treatment. B. Western blot showing reduction of c-Myc, ASCT2 and GLS expression in A2780/CP70 and OV81.2/CP10 in response to treatment with c-Myc inhibitor 10058-F4 (50uM). C. C-Myc inhibition using 50uM 10058-F4 decreased [H-3]GLN uptake in all cell lines, which is more pronounced in the CP70 and CP10 cells. Uptake is normalized to cell number. Experiments were performed in triplicate and repeated 3 times. Graphs present mean (boxes) and SD (bars; n=9).