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. 2016 May 12;7(27):41637–41649. doi: 10.18632/oncotarget.9317

Figure 4. GLS overexpression confers cisplatin resistance.

Figure 4

A. Western blot confirms over expression of glutaminase (GLS) in A2780 and OV81.2 cells similar to the expression level found in the resistant cell lines. B. A2780 GLS over expressing cells and A2780 expressing control LV05 vector and C. OV81.2 GLS over expressing cell and OV81.2 expressing control LV05 vector were treated with increasing concentrations of cisplatin. After 48 hr treatment cell viability was assessed by Vi-CELL trypan blue counter. Experiments were performed in duplicate and repeated 3 times. Graphs present mean (dots) and SD (bars; n=6) of percentage viable cells relative to untreated. Apoptosis was measured using annexin V staining in D. A2780 expressing control LV05 vector, A2780 GLS over expressing cells and CP70 cells, and E. OV81.2 expressing control LV05 vector, OV81.2 GLS over expressing cells and CP10 cells. Cells were incubated with and without cisplatin (10uM) for 72 hr. Experiments were performed in duplicate and repeated 3 times. Graphs present mean (boxes) and SD (bars; n=6) of total apoptosis. Total apoptosis is the sum of the percentage of annexin V only and annexin V / propidium iodide stained cells.