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. 2016 May 20;7(27):41650–41661. doi: 10.18632/oncotarget.9519

Figure 4. Lnc-CC3 knockdown suppressed migration and invasion in SiHa cells in vitro.

Figure 4

(A) Fold change in lnc-CC3 expression in SiHa cells was analyzed by qRT-PCR (n = 3); β-actin was used as an internal control. Data were analyzed using the 2−ΔΔCT method. (B) The shape of lnc-CC3 knockdown SiHa cells changed from elongated to round. Photograph at 100× magnification, scale bar = 200 μm. (C) Cell migration capacity was determined by wound healing assay (n = 3). Photographs were taken immediately at 0 h and 48 h after wounding at 100× magnification, at the same location in each well. (D) Representative images of the migration and invasion transwell assay; invaded cell number was determined by photograph at 200× magnification in five random views per chamber. Data are expressed as mean ± SD of independent experiments, *p < 0.05.