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. 2016 May 30;7(27):41767–41780. doi: 10.18632/oncotarget.9702

Figure 3. Posterior HOXD genes promote chondrogenic differentiation and expression of bone associated genes.

Figure 3

(A) Chondrogenic differentiation potential of ES lines with specific shRNA constructs was shown by the expression of specific chondrogenic marker genes COL10A1, IHH and SOX9 using qRT-PCR. Data are mean ± SEM; t-test. (B) Analysis of RUNX2 expression in A673, EW7 and SK-N-MC cells after transient combined HOXD knock down with specific siRNAs against HOXD10, HOXD11 and HOXD13 using qRT-PCR. Data are mean ± SEM; t-test. Middle panel western-blot of RUNX2 expression after triple HOXD knock down compared to respective controls (si.control: c). (C) mRNA analysis of BGLAP and PDGFB expression in 3 different ES lines after inhibition of HOXD10, HOXD11 or HOXD13 expression with specific shRNA. Data are mean ± SEM; t-test. (D) Expression analysis of PTHLH in ES after knock down of individual HOXD genes by qRT-PCR. Data are mean ± SEM; t-test.