Figure 6. HOXD11 and HOXD13 promote lung metastasis in ES.

(A) In vivo analysis of the metastatic potential of A673 cells constitutively transfected with sh.control, sh.HOXD10, sh.HOXD11 and sh.HOXD13 in Rag2^−/−γ_C^−/− mice (5 mice/group). Affected organs were photographed and analyzed by histology. Left, representative pictures of whole organs and H&E staining sections are shown (scale bar 5 mm). Right, average number of apparent metastases in lung and liver tissues is plotted. (B) Similar experiments were carried out with SK-N-MC cells constitutively transfected with sh.control, sh.HOXD10, sh.HOXD11 and sh.HOXD13 in immune deficient Rag2^−/−γ_C^−/− mice (5 mice/group). Here knock down of HOXD10 also inhibited lung metastasis (scale bar 5 or 1mm).